Additional file 4: of Deep sequencing of small RNA facilitates tissue and sex associated microRNA discovery in zebrafish

Comprises of barcharts for validation. Slide1: dre-miR-21 is found to be abundant and ubiquitously expressed as compared to some let-7 family members. The dre-let-7f, g, h appears to be enriched in female liver and dre-let7i is enriched in brain as compared to the embryo and other tissue samples. Slides 2 and 3 : shows the expression of the miRNAs 430 a,b,c in the embryo and the female tissues of gut, liver and ovary. The slides depict the overshadowing effect of high expression of these miRNAs in embryo over the female tissues. The high expression of these miRNAs in the female tissue is visible only when not compared to embryo. (PDF 227 kb