Knockdown of ALDH1A3 by shRNA inhibits invasion of glioma cells.

<p><b>A</b>. After transduction, ALDH1A3 expression was determined by western blot. The expression of ALDH1A3 was significantly reduced by shRNA targeting ALDH1A3 as measured by densitometry (normalized to GAPDH). <b>B</b>. The number of invaded cells was significantly decreased in ALDH1A3 knockdown cells as compared with control. <b>C</b>. The quantitative results for the transwell experiment. <b>D</b>. The expression of tumor invasion associated proteins was reduced by ALDH1A3 knockdown. Each experiment was performed in triplicates. * p<0.05.