EGFR phosphorylation is amplified in the immobile state.

<p>(<i>A</i>) Probability of colocalization between Cy3-SNAP-EGFR and EGFP-PTB. (<i>B</i>) Normalized intensity histograms of the Cy3-SNAP-EGFR intensity, for all particles (<i>continuous lines</i>) and for the particles that colocalized with EGFP-PTB (<i>dashed lines</i>) after 10 minutes of EGF stimulation. (<i>C</i>) Probability of colocalization between Cy3-SNAP-EGFR and EGF-Alexa488. (<i>D</i>) Fold-increase of the colocalization probability in the immobile versus the confined state, for EGFP-PTB colocalization (see <i>A</i>), and for EGF-Alexa488 colocalization (see <i>C</i>). <i>A</i>, <i>B</i>: <i>n</i> = 43 cells; <i>C</i>, <i>D</i>: <i>n</i> = 36 cells. ns: <i>P</i> > 0.05, **P < 0.01, ***P < 0.001, <i>t</i> test. Error bars denote SEM.