<p>JMY1877 recombinant strains transformed with the empty cassette or expressing AtDGAT1 or EgDGAT1-1 were grown for 18 h. FAs from twenty-five milligrams of dry cell lysate were transmethylated. The resulting FAMEs from three independent cultures were identified and quantified by GC-FID. (<b>A</b>) FA content values are expressed in percentage of dry cell weight per strain. Asterisks indicate statistically significant differences according to a <i>t</i>-test (*<i>P</i><0.05; **<i>P</i><0.01). (<b>B</b>) FA composition values are expressed as relative amount of each FA per strain. Asterisks indicate statistically significant differences according to a <i>t</i>-test (*<i>P</i><0.05; **<i>P</i><0.01; ***<i>P</i><0.001).</p
<p>A, Growth of wild-type (WT) and survival of <i>Fas2Δ/Δ</i> yeast cells in the indicated serum fra...
<p>(A) TLC analyses of the neutral lipids were performed as described previously [<a href="http://ww...
<p>Recombinant <i>S. cerevisiae</i> expressing empty pYES2 vector or Fads2 were grown in the presenc...
<p>H1246 strains transformed with the empty vector or expressing DGAT1<sub>opt</sub> or DGAT2<sub>op...
<p>JMY1877 recombinant strains expressing AtDGAT1 or EgDGAT1-1 were grown for 18 h in YPL medium. Li...
<p>The relative amount of fatty acids is expressed as a percentage of total fatty acid methyl esters...
<p>Total fatty acid content of GFP, AtOle1-GFP and AtClo1-GFP expressing cells after 18 or 42 h indu...
<p>A known amount of 18∶2 was added to cultures before induction with galactose and allowed to grow ...
<p>Cultures were induced with galactose and allowed to grow 28°C for 48 h (A) or at 15°C for 96 h (B...
<p>Results are expressed as a percentage of total FA substrate converted to desaturated products.</p
<p>All experiments were carried out in triplicate. Asterisks indicate statistically significant diff...
<p>Yeast cells were grown at 30°C in full medium (YP) supplemented with different carbon sources (gl...
The occurrence of protein synthesis errors (mistranslation) above the typical mean mistranslation le...
The occurrence of protein synthesis errors (mistranslation) above the typical mean mistranslation le...
<p>Results are expressed as a percentage of total fatty acid (FA) substrate converted to desaturated...
<p>A, Growth of wild-type (WT) and survival of <i>Fas2Δ/Δ</i> yeast cells in the indicated serum fra...
<p>(A) TLC analyses of the neutral lipids were performed as described previously [<a href="http://ww...
<p>Recombinant <i>S. cerevisiae</i> expressing empty pYES2 vector or Fads2 were grown in the presenc...
<p>H1246 strains transformed with the empty vector or expressing DGAT1<sub>opt</sub> or DGAT2<sub>op...
<p>JMY1877 recombinant strains expressing AtDGAT1 or EgDGAT1-1 were grown for 18 h in YPL medium. Li...
<p>The relative amount of fatty acids is expressed as a percentage of total fatty acid methyl esters...
<p>Total fatty acid content of GFP, AtOle1-GFP and AtClo1-GFP expressing cells after 18 or 42 h indu...
<p>A known amount of 18∶2 was added to cultures before induction with galactose and allowed to grow ...
<p>Cultures were induced with galactose and allowed to grow 28°C for 48 h (A) or at 15°C for 96 h (B...
<p>Results are expressed as a percentage of total FA substrate converted to desaturated products.</p
<p>All experiments were carried out in triplicate. Asterisks indicate statistically significant diff...
<p>Yeast cells were grown at 30°C in full medium (YP) supplemented with different carbon sources (gl...
The occurrence of protein synthesis errors (mistranslation) above the typical mean mistranslation le...
The occurrence of protein synthesis errors (mistranslation) above the typical mean mistranslation le...
<p>Results are expressed as a percentage of total fatty acid (FA) substrate converted to desaturated...
<p>A, Growth of wild-type (WT) and survival of <i>Fas2Δ/Δ</i> yeast cells in the indicated serum fra...
<p>(A) TLC analyses of the neutral lipids were performed as described previously [<a href="http://ww...
<p>Recombinant <i>S. cerevisiae</i> expressing empty pYES2 vector or Fads2 were grown in the presenc...