Anti-proliferative effects of CDDP, SAHA and VPA administered singly in three breast cancer cell lines (MCF7, T47D and MDA-MB-231) measured in vitro by the MTT assay.

<p>Results are presented as median inhibitory concentrations (IC₅₀ values in μg/ml ± S.E.M.) of CDDP, SAHA and VPA administered singly with respect to their anti-proliferative effects on three cancer cell lines (MCF7, T47D and MDA-MB-231) measured in vitro by the MTT assay. n—total number of items used at concentrations whose expected anti-proliferative effects ranged between 4 and 6 probits (16% and 84%); CFP–(q and p) curve-fitting parameters; q/p—ratio of q and p values; S.R.–slope function ratio (S_CDDP/S_SAHA, S_CDDP/S_VPA); f ratio S.R.–factor for slope function ratio. Test for parallelism of two DRRCs was performed according to Litchfield and Wilcoxon (1949). It this case, if the slope function ratio (S.R.) value is higher than the factor for slope function ratio (f ratio S.R.) value, the examined two DRRCs are not parallel to each other (Litchfield and Wilcoxon 1949). NP—not parallel; All detailed calculations required to test the parallelism of two DRRCs were presented in the Appendix to the paper by Luszczki and Czuczwar (2006).</p><p>Anti-proliferative effects of CDDP, SAHA and VPA administered singly in three breast cancer cell lines (MCF7, T47D and MDA-MB-231) measured in vitro by the MTT assay.