TIS-based identification of loci that interact genetically with <i>vchM</i> or <i>rpoE</i>.

<p>(A) For two comparative analyses (<i>∆vchM</i> vs. wt and <i>∆vchM ∆rpoE vs</i>. <i>∆vchM)</i>, the read count ratio of transposons inserted into each genetic locus was determined, and is plotted against the inverse of the associated p-value as determined by Mann-Whitney U analysis of read counts. Genes plotted above the horizontal line are considered significantly different in each analysis (p-value <0.001). (B) The raw number of reads originating from insertions on the forward (red) or reverse strand (green) in wt and <i>∆vchM</i> insertion libraries are shown for selected loci from both libraries. All potential insertion sites (TA dinucleotides) are marked by black bars. (C) The predicted σ^E stress response pathway in <i>V</i>. <i>cholerae</i>.