β-catenin deletion in DCs did not affect the cytokine production by CD4⁺ T cells in the steady state or during CIA.

<p>Representative FACS plots illustrating the pro-inflammatory cytokines IFNγ, IL-4, IL-17A and anti-inflammatory IL-10 produced by CD4⁺ T cells isolated from spleens of steady-state (left panel, n = 8 mice per group) and CIA (right panel, n = 10 mice per group) mice, on day 35 after the first immunization. Cells were stimulated for 4 hours with phorbolmyristate acetate (PMA) (0.05 μg/ml) and ionomycin (0.5 μg/ml) in the presence of GolgiStop. The frequencies of the different cytokine-secreting Th cells were similar in the β-cat^DEL and control groups in both conditions. Data are presented as mean ± SEM.