CHD8 knockdown in B-ALL cells leads to cell death without a cell cycle arrest.

<p><b>(A)</b> Flow cytometry plots showing induction of dsRed and a linked shRNA upon treatment with doxycycline. <b>(B)</b> Western blot showing decreased CHD8 expression over time following induction of sh<i>Chd8</i> in B-ALL cells by doxycycline. <b>(C)</b> Bar graph showing increased <i>CCNE1</i> and <i>CCNE2</i> expression upon CHD8 knockdown. Shown are averages ± SD. <b>(D)</b> Bar graph showing cell cycle profiles of B-ALL cells following shRNA induction by doxycycline. <b>(E)</b> Graph showing growth rates of B-ALL cells transduced with the indicated constructs. Shown are averages ± SD. <b>(F)</b> Bar graph showing percentages of dead cells following <i>Chd8</i> knockdown. Survival of cells transduced with sh<i>Chd8</i> may be due to selection against CHD8 knockdown. Shown are averages ± SD. *P < 0.01, **P < 0.005, ***P < 0.0005