Application of wobble correction for 2-color, 3D SR image registration.

<p>(a) A cartoon of the wobble effect in 2 independent channels is shown. Green and pink rectangles represent focal planes. Straight lines reference the wobble-free system. (b) Effect of merging channels: since the wobble differs over z, this increases the registration error <i>δ_z</i> where wobble curves differ most, which is typically at the z-limits of the imaging depth (c) the target registration error (TRE) as a function of z for a locally weighted mean (LWM) transformation defined at the focal plane (red data) and a LWM transformation defined at the focal plane with wobble correction included (black data). Error bars are standard deviation of TRE values. Measurements taken from 4 beads over the field of view.