Up-regulation of <i>OAS1</i> expression by HRV16.

<p>(<b>A</b>) Primary healthy (h) and CF (cf) HBE cells were pre-incubated for 18 hours with experimental culture medium (BEGM without hydrocorticortisone or antibiotics) and subsequently inoculated for 24 hours with HRV16 (HRV) at an MOI of 0.1. Afterwards, expression of <i>OAS1</i> mRNA was quantified by qPCR. Numbers on the plot represent <i>OAS1</i> expression in basal cells, assumed as 1. Other data are presented as box-and-whisker plots (medians, interquartile ranges, and min-max values) of fold up-regulation over basal. n = 8 cultures per group. ** p < 0.01 and *** p < 0.001. (<b>B</b>) Primary healthy (h) and CF (cf) HBE cells were pre-incubated as above and subsequently inoculated for 2 hours with HRV16 (HRV) at the MOI of 0.1. Then, virus-containing cell supernatants were removed, cells were rinsed twice with experimental culture medium to deplete extracellular virus, and incubated for 22 hours without HRV16. Afterwards, expression of <i>OAS1</i> mRNA was quantified by qPCR. Numbers on the plot represent <i>OAS1</i> expression in basal cells, assumed as 1. Other data are presented as box-and-whisker plots of fold up-regulation over basal. n = 6–7 cultures per group. * p < 0.05 and ** p < 0.01.