Real-time quantitative reverse transcription PCR analysis.

<p>The accession numbers and primer sequences of 16 genes associated with Chl biosynthesis and photosynthesis in IR64 and w67 are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143249#pone.0143249.s004" target="_blank">S3 Table</a>. The expression levels were determined by real-time PCR and normalized to the <i>ubiqutin</i>. The values are presented as the mean ± SD from three biological replicates. *, Significance at P ≤ 0.05; **, Highly significance at P ≤ 0.01.