The NOR-1 protector prevents the down-regulation of VCAM-1 promoted by miR-17 and -20.

<p>HUVEC were transfected with precursors of either miR-17 or miR-20a (P17 or P20) or a scramble sequence (Scr) in the presence or in the absence of a miScript target protector that blocks the NOR-1 3’-UTR seed sequence recognized by the miR-17 and -20a (NOR1-Prot). Then cells were stimulated with VEGF (100 ng/ml, 2 h). VCAM-1 expression was assessed by real-time PCR <b>(A)</b> and Western-blot <b>(B)</b>. Results are expressed as mean ± SD from at least n = 4. (<i>p</i><0.05: *, <i>vs</i>. untransfected cells [Control, CT] or cells transfected with Scr; #, <i>vs</i>. cells transfected with Scr and stimulated with VEGF; †, <i>vs</i>. cells exposed to the same condition but without NOR1-Prot).