Confirmation of selected transcript variants of a putative rainbowfish ERα cluster by endpoint reverse-transcriptase PCR (RT-PCR).

<p>(A) Schematic diagram of three transcripts in the cluster–<i>comp105150_c0_seq9</i>, <i>comp105150_c0_seq11</i> and <i>comp105150_c0_seq12</i>, showing binding sites for the PCR primers listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0142636#pone.0142636.t004" target="_blank">Table 4</a>. (B) Agarose gel electrophoresis of RT-PCR products generated using the indicated primer pairs. Primers targeting 18S rRNA were used to confirm the quality of cDNA in all samples. Abbreviations: kb, kilobases; M, marker; NT, no-template control; MB, male brain; MG, male gonad; ML, male liver; FB, female brain; FG, female gonad; FL, female liver; 18S, 18S rRNA.