Quantitative changes in splenic Treg and Th17 cell percentages and the relationship between Treg and Th17 subsets.

<p>Single-cell spleen suspensions from <i>C</i>. <i>sinensis</i>-infected and control mice (NC) were used to analyze Th17 and CD4⁺CD25⁺Foxp3⁺regulatory T cell percentages among CD4⁺ T cells by flow cytometry (<b>A</b>). Representative blots of IL-17⁺ and CD25⁺Foxp3⁺cells in splenic CD4⁺cell populations. <b>B</b> and <b>C</b> show quantitative changes in splenic Treg and Th17 cell percentages in CD4⁺T cells. The Treg/Th17 ratio was calculated (<b>D</b>). Data are expressed as mean ± S.E.M. Asterisks indicate statistically significant differences between infected and NC mice, **<i>P</i><0.01, ***<i>P</i><0.001, compared with normal control mice.