Radiolabeled ceramides with different acyl chain lengths metabolize into sphingolipids as a function of time.

<p>HeLa cells were loaded with C6-Cer <b>(A)</b>, C10-Cer <b>(B)</b> and C16-Cer <b>(C)</b> in CholPC complexes. The [³H]sphingosine labeled ceramides (50 μM) were allowed to be taken up by HeLa cells for 3, 6 and 24 hours, and the incorporation was followed by HPTLC analysis. The amount of each lipid is presented as a percentage of the total radioactivity in the sample. GSLs = GlcCer, GalCer, GlcCer-OH and GalCer-OH. Other = the remaining lane. The data is from at least three different experiments.