Inhibitory effects of kaempferol on tunicamycin-induced expression and activation of ATF6 and IRE1α (A), and GRP78 and HSP70 (B) in BEAS-2B cells.

<p>Cells were treated with 1–20 μM kaempferol and simulated with 1 μM tunicamycin. Cell lysates were prepared for Western blot analysis with a primary antibody against ATF6, phospho-IRE1α, GRP78 and HSP70. Representative blot data were obtained from 3 experiments, and β-actin protein was used as an internal control. The bar graphs (mean ± SEM) in the bottom panels represent quantitative results of upper blots. Values in bar graphs not sharing a letter indicate significant different at P<0.05.