Knocking down of TRIM59 arrests NSCLC cell cycle in G2 phase.

<p>HBE and H1299 cells were transiently transfected with TRIM59 siRNA-1, TRIM59 siRNA-2, control siRNA or untransfected were cultured in RPMI 1640 with 10% FBS for 48 hrs. <b>(A)</b> Adherent cells were collected and cell cycle analysis was done by flow cytometry. The inserts showed the proportion of cells for each phase and are marked with different colors (pink: G0/G1 phase, green: S phase, and gray: G2/M phase). <b>(B)</b> The ratio of the cells in each phase was counted. <b>(C)</b> H1299 cells were fixed and stained with anti-PCNA antibody (red) and DAPI (blue). <b>(D)</b>The protein expression levels of PCNA in H1299 cells were checked by western blot.