TRIM59 promotes the proliferation of NSCLC cells.

<p><b>(A)</b> Low serum assay. The indicated NSCLC cell lines transiently transfected with TRIM59 siRNA-1, TRIM59 siRNA-2, control siRNA or untransfected were cultured in RPMI 1640 medium supplemented with 1% FBS. At the indicated times, cells were trypsinized and counted. The data represent the average of three independent experiments (mean±SD) (Top figures). Immunoblot of TRIM59 to check the knockdown efficiency of TRIM59 siRNAs in the indicated cell lines (Bottom figures). <b>(B)</b> Saturation density assay. The indicated NSCLC cells transiently transfected with TRIM59 siRNA-1, TRIM59 siRNA-2, control siRNA or untransfected were cultured in RPMI 1640 supplemented with 10% FBS for 6 days, trypsinized and counted. The data represent the average of three independent experiments (mean±SD). <b>(C)</b> Colony formation assay. Five hundred H1299 cells transiently transfected with TRIM59 siRNA-1, TRIM59 siRNA-2, control siRNA or untransfected were seeded in 6-well plates in RPMI 1640 with 5% FBS. After two weeks, cells were fixed and stained with crystal violet. Representative wells were photographed and shown.