Phosphatidylserine and Spo14 localization confirm prospore membrane phenotypes.

<p>(A) Localization of phosphatidylserine (PS) in wild-type, <i>spo71</i>Δ, <i>spo73</i>Δ and <i>spo1</i>Δ cells. PS was detected using Lact-C2-GFP-p416 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143571#pone.0143571.ref003" target="_blank">3</a>]. Both elongated and rounded prospore membrane stages are shown for wild-type cells; elongated prospore membranes are not typically seen in <i>spo71</i>Δ and <i>spo73</i>Δ cells (B) Localization of Spo14 in wild-type, <i>spo71</i>Δ, <i>spo73</i>Δ and <i>spo1</i>Δ cells. Spo14 was detected using GFP-Spo14 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143571#pone.0143571.ref026" target="_blank">26</a>]. For <i>spo1</i>Δ, Class I represents cells without prospore membranes; Class II cells represents cells that make at least one prospore membrane, like in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143571#pone.0143571.g003" target="_blank">Fig 3</a>. n = 39.