Additional file 5: Construction of the Y. lipolytica strains in which the scGAL genes were overexpressed. The auxotrophic PO1d strain was used as the acceptor strain. The genes were inserted one by one to create strains that overexpressed different combination of the scGAL genes; URA3ex and LEU2ex were used as selection markers. To recover auxotrophies necessary to continuing the transformation process, a JME547 plasmid containing Cre-Lox recombinase was used to transform Y. lipolytica Y3683, thus generating strain Y3686 [35]. In turn, to recover prototrophy in the Y. lipolytica Y3687 strain, a purified SalI fragment of the pINA62 plasmid that contained the LEU2 gene was introduced [28]
Table S1. Six putative β-glucosidase coding genes identified harboring conserved glycosyl hydrolase...
Figure S1. Multiple alignments of putative conserved domains of the family 3 glycosyl hydrolases of ...
Additional file 2: Figure S1. The sketch map for knock-in of XI gene by one-step homologous recombin...
Additional file 2: Overexpression of scGAL and ylGAL genes in Y. lipolytica. Growth of Y. lipolytica...
Background :Production of valuable metabolites by Yarrowia lipolytica using renewable raw materials ...
Additional file 3: Table S1. Oligonucleotide primers used for Q-PCR. Table S2. Primers for PCR ampli...
Additional file 1: Figure S1. PCR verification of Y. lipolytica transformants expressing multiple ce...
Additional file 2. Candidate XYR genes in Y. lipolytica by BLAST analysis. A list of the putative XY...
Additional file 1: Figure S1. Amino acid sequence alignment of Yl-SPT15 (YALI0B23056g) in Y. lipolyt...
Additional file 1: Figure S1. (A) Growth of the strains ∆pox (square) and ∆pox∆YALI0F06754 (∆elo1) (...
Additional file 6: Fig. S2. GFP-based reporter system in Y. lipolytica. (a) Selection of functional ...
Additional file 4: Figure S2. The visualization of the PTKL1-gfp, PTAL1-gfp, PGDN1-gfp and PZWF1-gfp...
Additional file 7: Table S2. Oligonucleotides used in construct plasmids in this study. Table S3. Th...
Additional file 4. Oligonucleotides used in this study. A complete list of all of the oligonucleotid...
Additional file 3. Further information regarding the L. starkeyi genetic transformation system and...
Table S1. Six putative β-glucosidase coding genes identified harboring conserved glycosyl hydrolase...
Figure S1. Multiple alignments of putative conserved domains of the family 3 glycosyl hydrolases of ...
Additional file 2: Figure S1. The sketch map for knock-in of XI gene by one-step homologous recombin...
Additional file 2: Overexpression of scGAL and ylGAL genes in Y. lipolytica. Growth of Y. lipolytica...
Background :Production of valuable metabolites by Yarrowia lipolytica using renewable raw materials ...
Additional file 3: Table S1. Oligonucleotide primers used for Q-PCR. Table S2. Primers for PCR ampli...
Additional file 1: Figure S1. PCR verification of Y. lipolytica transformants expressing multiple ce...
Additional file 2. Candidate XYR genes in Y. lipolytica by BLAST analysis. A list of the putative XY...
Additional file 1: Figure S1. Amino acid sequence alignment of Yl-SPT15 (YALI0B23056g) in Y. lipolyt...
Additional file 1: Figure S1. (A) Growth of the strains ∆pox (square) and ∆pox∆YALI0F06754 (∆elo1) (...
Additional file 6: Fig. S2. GFP-based reporter system in Y. lipolytica. (a) Selection of functional ...
Additional file 4: Figure S2. The visualization of the PTKL1-gfp, PTAL1-gfp, PGDN1-gfp and PZWF1-gfp...
Additional file 7: Table S2. Oligonucleotides used in construct plasmids in this study. Table S3. Th...
Additional file 4. Oligonucleotides used in this study. A complete list of all of the oligonucleotid...
Additional file 3. Further information regarding the L. starkeyi genetic transformation system and...
Table S1. Six putative β-glucosidase coding genes identified harboring conserved glycosyl hydrolase...
Figure S1. Multiple alignments of putative conserved domains of the family 3 glycosyl hydrolases of ...
Additional file 2: Figure S1. The sketch map for knock-in of XI gene by one-step homologous recombin...