Osteoblast-like MG-63 cells attach on the CS5 scaffold.

<p>The high affinity of human bone cells indicated that the fabricated biocomposites act as a biomimic of the human bone scaffold. Live color staining and fluorescence graphs of Nuclear/Actin contact analysis are shown in upper panel. (a) unattached scaffold, (b) live cell staining of formazan on scaffold, and (c) fluorescence on scaffold. The live cells on scaffolds were incubated in a tetrazolium dye bath at 37°C for 4 h. Green signals indicate actin, and blue signals indicate the nuclear site of cells. (d). CS5 scaffolds showing a long-term survival period of 1–6 days. Cell numbers corresponding to the OD₅₇₀ values represented approximately 836 ± 37 cells for each 0.1 OD value by MTT assay, for normalization with cell counting. ** P<0.01 compared to day 1 group.