Release of the chemoattractant Ccl2/MCP1 in untreated Ifnar^del mice.

<p>(A) qRT PCR analysis of mRNA levels of the chemokines <i>Cxcl9</i>, <i>Cxcl10</i>, <i>Cxcl11</i>, <i>Ccl2</i>, <i>Ccl4</i>, <i>Ccl5</i> and <i>Ccl25</i> from whole pancreatic tissue of WT and Ifnar^del mice harvested 4 hours following caerulein-induced injury (n = 3 per group. Bars indicate mean +/- SD. Normalized on the mRNA of the housekeeping gene <i>Ppib</i>). (B) qRT PCR analysis of mRNA levels of the chemokines <i>Cxcl9</i>, <i>Cxcl10</i>, <i>Cxcl11</i>, <i>Ccl2</i>, <i>Ccl4</i>, <i>Ccl5</i> and <i>Ccl25</i> from whole pancreatic tissue of untreated WT and Ifnar^del mice (n = 7 per group. Bars indicate mean +/- SD. Normalized on the mRNA of the housekeeping gene <i>Ppib</i>. *P<0.05, unpaired Student´s t-test). (C) Immunohistochemical staining for MCP1-positive centro-acinar cells in the pancreas from WT and Ifnar^del mice 24 hours following caerulein-induced injury or untreated and counting of the absolute number of positive cells on five separate high power fields for each section of untreated WT and Ifnar^del mice and after 24 hours following caerulein-induced injury (n = 3–7 per group. Bars indicate mean +/- SD. ****P<0.0001, Mann-Whitney-test. Original magnification, 200x and 1000x).