Serum antibodies to canine P450scc in radioimmunoprecipitation assay.

<p>(A) Scatter plot from a representative 96-well plate showing P450scc immunoreactivity (counts per minute; CPM, mean of triplicate wells) in serum samples from hospital control dogs (Control; <i>n</i> = 11) and dogs affected with hypoadrenocorticism (HypoAd; <i>n</i> = 19). Negative standard (triangle) and positive standard (square) serum samples were identified from initial autoantibody screening experiments and were used on every plate for normalisation purposes. (B) Relative P450scc autoantibody reactivity in control dogs (<i>n</i> = 80), dogs affected with spontaneous hypoadrenocorticism (HypoAd; <i>n</i> = 213), dogs affected with hyperadrenocorticism (HAC; <i>n</i> = 58) and dogs with iatrogenic hypoadrenocorticism (iHAd; <i>n</i> = 4). Circles represent normalised values for each individual serum sample in the P450scc radioimmunoprecipitation assay. Mean + 3SD line represents the threshold positive value established from a reference population of 30 canine control patients.