Knockdown of enzymes of the SUMOylation pathway does not alter expression of a stably integrated CMV-eGFP gene.

<p>A HeLa cell line stably expressing eGFP was transfected with four different siRNAs targeting Ubc9 and Sae2, respectively. Forty-eight h later, the cells were infected with scAAV2-renilla luciferase at an MOI_GC of 10³. Twenty-four h after infection, cells were harvested by trypsinization. One half of a well of a 6-well plate was proceeded for FACS-analysis (a), the other half was used to determine luciferase activity (b). Mean values and standard deviation of two independent experiments of mean fluorescent intensity (MFI) and relative light units (RLU), respectively, are shown. The values obtained after transfection with <i>AllStars negative control siRNA</i> (Qiagen; ‘scrambled’) were set to 100 in both cases.