Analysis of reverse transcription by wild type or RT-W252A mutant virus in Jurkat cells.

<p>¹. The infection experiments were repeated at least three times and the mean copy numbers of DNA measured and standard deviations are shown;</p><p>². HIV-1NL4.3;</p><p>³. HIV-1NL4.3-RT-W252A;</p><p>⁴. Heat inactivated virus samples measured less than 100 copies (subtracted);</p><p>⁵. The data sets analyzed were considered to be normal with an α value of > 0.05 standard deviations. P values from Welch’s two-tailed t test are shown, comparing experimental and control samples as indicated;</p><p>⁶. Nevirapine (NVP) was added at 40 nM which is the reported IC50 in cell culture;</p><p>⁷. NT = Not Tested;</p><p>⁸. CA measured by ELISA;</p><p>⁹. RT measured by <i>in vitro</i> RT assay.</p><p>Analysis of reverse transcription by wild type or RT-W252A mutant virus in Jurkat cells.