CCHFV strain IbAr10200 replication machinery does not significantly discriminate against minigenomes from other CCHFV strains.

<p>(A) Absolute NanoLuc signal in relative light units (RLU) in BSR-T7 cells transfected with IbAr10200 NP, L, and GPC plasmids, and with minigenomes encoding L NCR of other CCHFV strains, as indicated. Data are reported as standard error of the mean (n = 8 from 2 experiments). (B) TCID₅₀ determination in SW-13 cells treated with supernatants from cells in (A). Results from 2 experiments are shown. (C) NanoLuc signal in SW-13 cells treated as in (B) (n = 8 from 2 experiments). NanoLuc signal data in VLP-treated cells are presented as absolute RLU values which are calculated as signal in SW-13 cells treated with entry-competent VLPs (i.e., containing NP, L, and GPC) minus signal in SW-13 cells treated with VLPs containing only NP, L and the corresponding minigenome. All data are reported as standard error of the mean.