Sequence alignment of the first extracellular domain of Cx50 and Cx36.

<p>The first extracellular domains (E1) of mouse Cx50 (42–74) and Cx36 (43–75) are aligned. Ten out of thirty-three (10/33) residues are different between these two connexins, including four residues with charge changes (asterisks), G46D, D51M, E62N and E68R. The naming of these mutants was according to the residue number of Cx50. Negatively charged residues (red) and positively charge residue (blue) are highlighted.