The inhibitory effect on miR-223 activity by dTuD-miR-223.

<p>(A) Schematic representation of 3′UTR luciferase reporter plasmid in which the 3′UTR of RhoB contained the binding sites of miR-233 (1726–1283 bp from 5′end) was fused to the 3′end of <i>Firefly Luciferase</i> (FLuc) after stop codon. (B) Dual-luciferase assay in 293A cells co-transfected with multiple plasmids as indicated. H1-TuD-miR-223 and U6-TuD-miR-223 represent the modified dTuD vector harboring a single TuD-miR-223 expression cassette driven by H1 and U6 promoter, respectively. After performing dual luciferase assay, the ratio FLuc/<i>Renilla</i> Luc (RLuc) was normalized to that of dTuD-Ctrl. (C) Expression level of miR-223 or (D) <i>RhoB</i> mRNA in 293A cells transfected with antagomir, TuD or dTuD against miR-233. Data were represented as the mean ± SD (n = 3). <i>* p</i> < 0.05, *** <i>p</i> < 0.001.