Plasmids for the expression of recombinant VP1 antigen and western blotting of expressed WCFS1-pSIP411-VP1 and NC8-pSIP411-VP1 proteins.
- Miao Wang (248377)
- Li Pan (49390)
- Peng Zhou (116747)
- Jianliang Lv (832543)
- Zhongwang Zhang (339465)
- Yonglu Wang (284548)
- Yongguang Zhang (318583)
Publication date
December 2015
DOIAbstract
<p>(A) The pSIP411-VP1 plasmid was constructed as described in the article and the purple arrow stands for optimized VP1. (B) The VP1 proteins expressed in WCFS1-pSIP411-VP1 and NC8-pSIP411-VP1 were detected by western blotting. Lane 1: WCFS1-pSIP411 induced by SppIP; Lane 2: WCFS1-pSIP411-VP1 without inducer; Lane 3: WCFS1-pSIP411-VP1 induced by SppIP; Lane 4: NC8-pSIP411-VP1 induced by SppIP; Lane 5: FMDV as positive control; Lane 6: NC8-pSIP411-VP1 without inducer.; Lane 7: NC8-pSIP411 induced by SppIP. Abbreviations: sh71rep: replication origin for lactobacillus; ermL: erythromycin-resistance marker; PsppIP: inducible promoters; sppK: histidine protein kinase; sppR: response regulator.</p