<i>L</i>. <i>major</i> strain FV1 metacyclic promastigotes and LPG stimulate <i>IL12B</i> expression.

<p>(<b>A</b>) Human DCs were infected with <i>L</i>. <i>major</i> FV1 or <i>L</i>. <i>major</i> LV39c5 parasite strains (n = 4 donors). After 8 hours, RNA was extracted from infected hDCs for cDNA generation and analyzed for <i>IL12B</i> expression by qRT-PCR. All values were significantly greater than uninfected. (<b>B</b>) Human DCs were infected with <i>L</i>. <i>major</i> FV1 metacyclic promastigotes (metacyclic), procyclic promastigotes (procyclic) or amastigotes (n = 3 donors). After 8 hours, RNA was extracted from infected hDCs for cDNA generation and analyzed for <i>IL12B</i> expression by qRT-PCR. All values were significantly greater than uninfected, except for amastigote infections. (<b>C</b>) Human DCs were exposed to different concentrations of LPG (0.5, 1, and 10 μg), derived from <i>L</i>. <i>major</i> FV1 metacyclic promastigotes (n = 4 donors). After 8 hours, RNA was extracted from infected hDCs for cDNA generation and analyzed for <i>IL12B</i> expression by qRT-PCR. Fold change was calculated utilizing the ΔΔC_T method and depicted as fold change over uninfected samples. Box plots display the median value (line), the interquartile range (box), and Tukey whiskers encompassing data within 1.5 fold of the interquartile range. *Statistical significance as compared to uninfected control, (p<0.05). All values were significantly greater than uninfected.