Comparison of the tandem mass spectra of a fucosylated and non-fucosylated glycopeptide originating from the glycosylation site at N360.

<p>(A) Positive ESI fragment ion spectrum of the peptide FYAGNITCR modified with the N-glycan of the composition (HexNAc)₂(Hex)₂. The [M+2H]²⁺ precursor at 916.394 m/z was fragmented with HCD at a normalized collision energy of 30. The peptide y ion series as well as the relevant fragment ions for the identification of the N-glycan are indicated. (B) Positive ESI fragment ion spectrum of the peptide FYAGNITCR modified with the N-glycan of the composition (HexNAc)₂(Hex)₂(Fuc). The [M+2H]²⁺ precursor at 989.423 m/z was fragmented with HCD at a normalized collision energy of 30. The peptide y ion series as well as the relevant fragment ions for the identification of the N-glycan are indicated.