No impact of <i>Thbs4</i>-deficiency on bone mass or matrix mineralization on a wildtype or <i>Hyp</i> genetic background.

<p>(A) Von Kossa/van Gieson staining of spine sections from 6 and 26 weeks old wildtype (+/+) and <i>Thbs4</i>-deficient (-/-) mice. (B) Quantification of the trabecular bone volume per tissue volume (BV/TV) and trabecular thickness (Tb.Th.) in wildtype (WT) and <i>Thbs4</i>-deficient (KO) mice at both ages. Bars represent mean ± SD (n = 6 per group). (C) Xray analysis (top panels) and von Kossa/van Gieson staining of tibia (middle panels) or spine sections (bottom panels) from 6 weeks old Hyp mice with (+/+) or without (-/-) <i>Thbs4</i>. (D) Quantification of the growth plate width (GPW) in the tibia (top) or the osteoid volume per bone volume (OV/BV) from mice of the indicated genotypes. Bars represent mean ± SD (n = 5 per group). Asterisks indicate statistically significant differences towards WT controls (p<0.05).