C-banding (a, d), CMA₃ fluorochrome staining (b, e) and FISH of the <i>C</i>_<i>0</i><i>t</i>-DNA (c, f) on mitotic chromosomes of <i>G</i>. <i>assimilis</i> (a, b, c) and <i>E</i>. <i>surinamensis</i> (d, e, f) cells.

<p>The sex chromosomes and the chromosomes bearing the secondary constrictions (pair 1) are indicated in each of the panels. Note the predominance of terminal C-positive blocks in <i>G</i>. <i>assimilis</i> and <i>E</i>. <i>surinamensis</i> chromosomes, the neo-Y chromosome with some heterochromatic blocks and CMA₃⁺ signals along its entire length, as well as the <i>C</i>_<i>0</i><i>t</i>-DNA signals. In (b), the white arrow indicates the interstitial CMA₃⁺ block, and in (d), the black arrow indicates the interstitial C-positive block in the neo-X₂ chromosome. In (e) arrowheads indicate the CMA₃⁺ signals in C-negative regions of the neo-X₁ and neo-X₂. One chromosome is missing in (b). Bar = 5 μm.