Additional file 2: of SJP-L-5, a novel small-molecule compound, inhibits HIV-1 infection by blocking viral DNA nuclear entry

Subcellular localization of HIV-1 IN, MA, and Vpr in transfected cells. Figure S1. Subcellular distributions of HIV-1 IN, MA and Vpr in transfected cells. (A). Subcellular distributions of HIV-1 IN, MA, and Vpr in control cells. 293 T cells were transiently transfected with 0.2 μg of the plasmids pC1-GFP, pC1-GFP2-NLS, pGFP-IN, pGFP-MA, or pGFP-Vpr using Lipofectamine 2000 reagent, the primers used in plasmid constructions were shown in Additional file 1: Table S1. At 48 h post transfection, the cells were fixed with 4 % paraformaldehyde (w/v) and then stained with DAPI. (B-D). Subcellular distributions of HIV-1 IN, MA, and Vpr in SJP-L-5 treated cells. 293 T cells were transiently transfected with 0.2 μg of the plasmids pGFP-IN (B), pGFP-MA (C), or pGFP-Vpr (D) using Lipofectamine 2000 reagent in the presence of 5 μg/ml or 20 μg/ml of SJP-L-5. At 48 h post transfection, the cells were fixed with 4 % paraformaldehyde (w/v) and then stained with DAPI. GFP (Green) and DAPI (Blue) fluorescence were observed using a confocal microscope. (TIFF 1744 kb