Additional file 5: Figure S4. of Impaired aldehyde dehydrogenase 1 subfamily member 2A-dependent retinoic acid signaling is related with a mesenchymal-like phenotype and an unfavorable prognosis of head and neck squamous cell carcinoma

(A) Representative phase contrast pictures of FaDu, Detroit562 and UMSCC-17B cells, which were treated with DMSO, 3 μM WIN18.446 or 3 μM BMS493 for four days. (B) Representative fluorescent pictures of FaDu and Detroit562 cells, which were treated as described in (A), and were stained with Phalloidin-Alexa488 (green signal). Nuclear staining was done with H33342 (blue signal). Migration of Detroit562 (C) and FaDu cells (D), which were treated with DMSO (white bars), 3 μM WIN18.446 (grey bars) or 3 μM BMS493 (black bars), in a scratch wounding assay was determined by the relative gap closure at the indicated time points. * p value ≤ 0.05. (TIF 4129 kb