Increased expression of some toxins in macrophages.

<p>mRNA was isolated from <i>Salmonella</i>-infected bone marrow derived macrophages pre-treated with IFNγ. The level of toxin mRNA was monitored by semi-quantitative RT-PCR and fold change relative to 16S RNA is shown. mRNA from <i>relE-4</i> was not detected. Mean of <u>></u> 3 independent experiments performed in triplicate are shown; error bars denote SEM. Two tailed Student’s <i>t-test</i> comparing macrophage expression to expression in DMEM, <i>P <</i> 0.05 (*), <i>P <</i> 0.0001 (***). Growth of <i>S</i>. Typhimurium in DMEM and LB were equivalent.