Effect of sulfur starvation on the transcription of sulfur metabolism genes of <i>A</i>. <i>flavipes</i>.

<p>Sulfur starved and non-starved spores of <i>A</i>. <i>flavipes</i> were transferred to basal Dox’s medium containing MgSO₄, L-methionine, cysteine, and cystine. Total RNA from the fungal cultures of these treatments was reverse transcribed and used as template in Reverse Transcription (RT)-qPCR using gene-specific primers for the indicated enzymes. Transcription levels were normalized to the transcription of constitutively expressed Actin A gene. Fold changes in transcription of starved cultures were calculated relative to non-starved controls.