Identification of <i>VdCYC8</i>-complemented mutants in <i>Verticillium dahliae</i> by PCR identification and <i>VdCYC8</i> relative expression analysis.

<p><b>A)</b> Confirmation of the presence of the wild-type complemented <i>VdCYC8</i> fragment. <b>B)</b> Confirmation of the presence of the chlorimuron cassette in the Δ<i>CYC8</i>-C strains. Lanes 1–15 indicate PCR results using primer Sul-F/R and DNA template obtained from the different complemented mutant strains, respectively; Lane 16 represents T286. <b>C)</b> Relative expression analysis of <i>VdCYC8</i> using primer CYC8RT-F/R. *Indicates a significant difference between mutant and the wild-type strain Vd080 (P<0.05).