Chronic exposure of mouse primary PAC to nicotine reduces the levels of TPKase proteins (A) and mRNA (B).

<p>Western blot analysis was carried out using pancreatic acinar whole cell proteins (60 μg) isolated from chronic nicotine exposed mice and their controls. Real-time PCR was performed using mice gene-specific primers. Data are mean ± SE from separate sets of samples from multiple mice and were normalized relative to ARPO and calculated by the relative relationship method. *<i>P</i> < 0.01, **<i>P</i> < 0.05.