Cytokine production in LX-2 cells.

<p>(A) Effects of LPS stimulation on the expression of cytokine mRNA. LX-2 cells were treated with or without 100 ng/mL LPS for 24 hours. Gene expression levels of IL-6, MCP-1 and IL-1β were examined by real-time RT-PCR. GAPDH was used for normalization. (B) Effects of LPS stimulation on the expression of cytokines at protein level. IL-6 and MCP-1 protein levels were determined by ELISA. Results are expressed as mean ± SD. Minimum three replicates were performed for each set of experiments to compile the data as presented.