<p>(A) Schematic diagram of mutant and wild type of HbFd1. HbFd1△C1 represents deletion of 10 amino acids in the C-terminus of wild-type HbFd1, and HbFd1△C2 means deletion of 20 amino acids in its C-terminus. (B) Investigation of the interaction between HbCIPK2 and mutant HbFd1. 10-fold dilutions (A<sub>600</sub> of 1–10<sup>−4</sup>) of the yeast Mav203 strain harboring the bait vector and the indicated prey construct were spotted on selection media without Leu and Trp (selection for positive transformants) or without Leu, Trp, His and Ura (with 35 mM 3-amino-1,2,4-triazole; selection for interaction) and incubated for 3–5 days at 30°C. Meantime, the interaction was confirmed by ß-Gal assays. The growth of cells transformed with wild-type ...
<p>(A) Bait vectors expressing either a catalytically inactive variant of the wild-type SLT-1 A<sub>...
<p>(A) Yeast cells co-transformed with the indicated combinations of JAZ-BD (pGBKT7) and bHLH-AD (pG...
In yeast hybrid assays, the process of identifying preys that interact with the bait of interest inv...
<p>The coding sequence for full length wild type SIRT6 and the H133W mutant was PCR-amplified and cl...
<p>AH109 yeast host cells were co-transformed with the indicated combinations of plasmids and grown ...
<p>(A) A yeast two-hybrid assay showing interaction between Gm08DWD and GmMYB176 Yeast cells were co...
<p>Serial dilutions of yeast cells (cells ml<sup>-1</sup>) that were transferred with the prey and b...
<p>(A) Yeast two-hybrid assay. The combinations of the plasmids used for transformation of the yeast...
(A) Representative yeast colonies transformed with wild-type Cbl or RF Mutant Cbl C381A as the bait ...
<p>Yeast two-hybrid data for Lhx3 (constructs comprised both LIM domains), the Lhx3 phosphomimic mut...
<p>(A) Schematic representation of the different Gal4-DBD-PR-Set7 bait proteins used for the yeast t...
<p>+ and − signs to the right of the yeast colonies indicate positive or negative interaction, respe...
<p>(A) An illustrative yeast two hybrid Interaction assay involving wild type PipX, P<sub>II</sub>, ...
<p>The bait was the CMV-P1 RNA1 helicase domain. For prey, PMM, Phosphomannomutase; FDH, Formate deh...
<div><p><b>A</b>: Peptide sequences of Aß alleles. </p> <p>Amino acid substitutions in mutated allel...
<p>(A) Bait vectors expressing either a catalytically inactive variant of the wild-type SLT-1 A<sub>...
<p>(A) Yeast cells co-transformed with the indicated combinations of JAZ-BD (pGBKT7) and bHLH-AD (pG...
In yeast hybrid assays, the process of identifying preys that interact with the bait of interest inv...
<p>The coding sequence for full length wild type SIRT6 and the H133W mutant was PCR-amplified and cl...
<p>AH109 yeast host cells were co-transformed with the indicated combinations of plasmids and grown ...
<p>(A) A yeast two-hybrid assay showing interaction between Gm08DWD and GmMYB176 Yeast cells were co...
<p>Serial dilutions of yeast cells (cells ml<sup>-1</sup>) that were transferred with the prey and b...
<p>(A) Yeast two-hybrid assay. The combinations of the plasmids used for transformation of the yeast...
(A) Representative yeast colonies transformed with wild-type Cbl or RF Mutant Cbl C381A as the bait ...
<p>Yeast two-hybrid data for Lhx3 (constructs comprised both LIM domains), the Lhx3 phosphomimic mut...
<p>(A) Schematic representation of the different Gal4-DBD-PR-Set7 bait proteins used for the yeast t...
<p>+ and − signs to the right of the yeast colonies indicate positive or negative interaction, respe...
<p>(A) An illustrative yeast two hybrid Interaction assay involving wild type PipX, P<sub>II</sub>, ...
<p>The bait was the CMV-P1 RNA1 helicase domain. For prey, PMM, Phosphomannomutase; FDH, Formate deh...
<div><p><b>A</b>: Peptide sequences of Aß alleles. </p> <p>Amino acid substitutions in mutated allel...
<p>(A) Bait vectors expressing either a catalytically inactive variant of the wild-type SLT-1 A<sub>...
<p>(A) Yeast cells co-transformed with the indicated combinations of JAZ-BD (pGBKT7) and bHLH-AD (pG...
In yeast hybrid assays, the process of identifying preys that interact with the bait of interest inv...