Additional file 1: Figure S1. of Down-Regulation of Cytokinin Oxidase 2 Expression Increases Tiller Number and Improves Rice Yield

Expression of OsCKX2 transcript in different rice organs of untransfomed wild type (WT) and in the young stems of CX3- and CX5-suppression lines, as assayed by RT-PCR (A, B) and by northern blot analysis (C). (A) Total RNA was isolated from leaf (L), root (R), stem (S), inflorescence (I), and green floret (F) of untransformed WT. (B) Total RNA was isolated from the young stems of untransformed WT and selected T1 transgenic lines. Expression of actin was included as a cDNA loading control. (C) RNA gel blot analysis of OsCKX2 transcript in the young stems of untransformed WT and selected T1 transgenic lines. Total RNAs isolated from transgenic lines harboring shRNA-CX3 (C-a), and shRNA-CX5 (C-b) were hybridized with a 32P-labelled OsCKX2 probe. Ethidium bromide staining of rRNA indicated an equal loading of RNA in each sample. (DOC 241 kb