<p>PBMC were first treated with medium (white points) or primed with 10 ng/mL LPS (black points), then cells were stimulated with 0.1 to 4 μg/mL PHA (<b>A</b>), or 63x10<sup>3</sup> to 500x10<sup>3</sup> anti-CD2/CD3/CD28-coated beads /mL (<b>B</b>), or 5 to 50 ng/mL OKT3 (<b>C</b>) for 72h. T cell proliferation was measured by flow cytometry and results are expressed as percentages of proliferating cells among total T cells. Each experiment was performed on <i>n</i> = 4 HV. Technical replicates were summarized by the mean for each individual. For each condition, individual values are summarized on the plots by a horizontal line representing the mean of a given condition.</p
<p>Spleens were harvested at D5 and splenocytes were rapidly isolated in order to determine T cells ...
<p>(<b>A</b>) Alloreactive T cell proliferation after addition of 25 µM kyn to mixed leukocyte react...
<p>(<b>A–B</b>) The frequency of activated CD38+HLA-DR+ T cells in VNPs and PPs by flow cytometry: (...
<p>PBMC were primed overnight with LPS concentrations ranging from 0.01 ng/mL to 100 ng/mL (in case ...
<p>PBMC (open diamonds) and T cells (<b>A</b>—black diamonds) or monocyte depleted PBMC (<b>B</b>- b...
<p>PBMC were treated or not overnight with 10 ng/mL LPS, washed and then treated simultaneously or n...
<p>PBMC were treated with medium or 10 ng/mL LPS and flow cytometry phenotyping was performed every ...
<p>(A) T cell enriched PBMCs were pretreated with LA prior to stimulation with anti-CD3/CD28 for 72 ...
<p>PBMC were primed overnight with 1 ng/mL, 10 ng/mL or 100 ng/mL LPS. Then PBMC were stimulated wit...
<p>T-cell proliferation is dependent on cell-cell contact to monocytes whereas NK-cell proliferation...
<p>Subconfluent monolayers of HCT-116 cells were FBS depleted overnight and treated with LPA at the ...
B cells were isolated and stimulated with CD40L/LPS/CpG for 2 days. Unstimulated (US) and stimulated...
<p>The extent of proliferation was calculated after 72-culture from the decrease in fluorescence per...
<p>PBMC were stimulated with either UL138 or gB peptides for 48(control). PBMC were then stimulated ...
<p>PBMCs were stained with the intracellular dye CFSE and the number of divisions which proliferatin...
<p>Spleens were harvested at D5 and splenocytes were rapidly isolated in order to determine T cells ...
<p>(<b>A</b>) Alloreactive T cell proliferation after addition of 25 µM kyn to mixed leukocyte react...
<p>(<b>A–B</b>) The frequency of activated CD38+HLA-DR+ T cells in VNPs and PPs by flow cytometry: (...
<p>PBMC were primed overnight with LPS concentrations ranging from 0.01 ng/mL to 100 ng/mL (in case ...
<p>PBMC (open diamonds) and T cells (<b>A</b>—black diamonds) or monocyte depleted PBMC (<b>B</b>- b...
<p>PBMC were treated or not overnight with 10 ng/mL LPS, washed and then treated simultaneously or n...
<p>PBMC were treated with medium or 10 ng/mL LPS and flow cytometry phenotyping was performed every ...
<p>(A) T cell enriched PBMCs were pretreated with LA prior to stimulation with anti-CD3/CD28 for 72 ...
<p>PBMC were primed overnight with 1 ng/mL, 10 ng/mL or 100 ng/mL LPS. Then PBMC were stimulated wit...
<p>T-cell proliferation is dependent on cell-cell contact to monocytes whereas NK-cell proliferation...
<p>Subconfluent monolayers of HCT-116 cells were FBS depleted overnight and treated with LPA at the ...
B cells were isolated and stimulated with CD40L/LPS/CpG for 2 days. Unstimulated (US) and stimulated...
<p>The extent of proliferation was calculated after 72-culture from the decrease in fluorescence per...
<p>PBMC were stimulated with either UL138 or gB peptides for 48(control). PBMC were then stimulated ...
<p>PBMCs were stained with the intracellular dye CFSE and the number of divisions which proliferatin...
<p>Spleens were harvested at D5 and splenocytes were rapidly isolated in order to determine T cells ...
<p>(<b>A</b>) Alloreactive T cell proliferation after addition of 25 µM kyn to mixed leukocyte react...
<p>(<b>A–B</b>) The frequency of activated CD38+HLA-DR+ T cells in VNPs and PPs by flow cytometry: (...