Effects of the addition of berberine to 5-ASA on PGE2 production and TNF-α secretion of lymphocytes.

<p>Experimental colitis was induced by giving mice three cycles of DSS. Each cycle consisted of 2% DSS for five days followed by drinking water for 14 days. Control mice received water alone. At the end of treatment, mice were killed and the lymphocyte were isolated from spleens and then cultured for the measurement of PGE2 production and TNF-α secretion without (A and B) or with PMA/ionomycin re-stimulation (C and D). ^a <i>P</i> < 0.05,^c <i>P</i> < 0.001 versus water mice; ^e <i>P</i> < 0.05, ^f <i>P</i> < 0.01 versus vehicle control (<i>n</i> = 3).