Sod2 is repressed in ESCs subjected to distinct differentiation protocols.

<p>ESCs were cultured as described in each case. Then, RNA was extracted and the expression of the indicated genes was measured by RT-qPCR. Gene expression was normalized to the geometrical mean of Gapdh and Pgk1 expression and referred to the control condition. Results are shown as mean ± SEM of three independent experiments. <b>(A)</b> R1 ESCs were cultured under standard conditions in the presence of LIF (control, shown as a dashed line) or in the absence of LIF, for 4 days. * p < 0.05. <b>(B)</b> 46C ESCs were subjected to a neural progenitor differentiation protocol. Expression of the indicated genes was analyzed at days 0 (D0, control), 3 (D3) and 6 (D6) after the induction of differentiation. Different letters (A or B) indicate statistically significant differences between treatments. AB indicates no statistically significant difference either to A or to B (p < 0.05).