AMPK activation contributes to the induction of UCP2 and the down-regulation of MPC1.

<p><b>(A)</b> Western blot analysis of phospho-Thr172-AMPKα in shGTPBP3-1, shGTPBP3-2 and negative control (NC) cells treated (+) or not (-) with AMPKα activator AICAR (1 mM) for 1 h or with AMPKα inhibitor Compound C (5 μM) for 1 h. The filter was also probed with AMPKα. <b>(B)</b> qRT-PCR analysis of <i>UCP2</i> mRNA expression in shGTPBP3-1, shGTPBP3-2 and NC cells treated or not with AICAR (1 mM) for 1 and 48 h or with Compound C (CC, 5 μM) for 1 h. <b>(C)</b> qRT-PCR analysis of <i>MPC1</i> mRNA expression in shGTPBP3-1, shGTPBP3-2 and NC cells treated or not with AICAR (1 mM) or with Compound C (CC, 5 μM), both for 1 h. All data are the mean ± SEM of at least three independent biological replicates. Differences were found to be statistically significant at *p<0.05, **p<0.01, ***p<0.001. n.s.: non-significant differences, A.U.: arbitrary units.