ATP levels and Complex I activity are reduced in shGTPBP3 cells whereas AMPK is activated.

<p><b>(A)</b> ATP levels in shGTPBP3-1, shGTPBP3-2 and negative control (NC) cells. <b>(B)</b> Measurement of mitochondrial Complex I, II, IV and V activities. All activities are expressed as citrate synthase ratios ((nmol/min/mg protein)/(citrate synthase-specific activity) x 100). <b>(C)</b> Western blot analysis of phospho-Thr172-AMPKα in shGTPBP3-1, shGTPBP3-2 and NC cells. The filter was also probed with AMPKα as a loading control. <b>(D)</b> Densitometric analysis of phospho-Thr172-AMPKα normalized to the loading control and represented as % of NC. All data are the mean ± SEM of at least three independent biological replicates. Differences from NC values were found to be statistically significant at *p<0.05, **p<0.01 and ***p<0.001.