Intracellular processing of the fusion protein ACP-GFP-Histag by the protease TEV coexpressed in <i>E</i>. <i>coli</i>.

<p><i>E</i>. <i>coli</i> cells were transformed with vector pSU18-MBP-TEV-WHZ and pET-28b-ACP-GFP-Histag respectively, or co-transformed with vector pSU18-MBP-TEV-WHZ and pET-28b-ACP-GFP-Histag. After induced by addition of IPTG to 0.4 mM in the medium for 12 h at 20°C, the <i>E</i>. <i>coli</i> cells were lysed by sonication on ice. After that, the cell lysate was centrifuged to remove cell debris, then the supernatants were recovered and the insoluble fraction was assumed to consist of inclusion bodies. W: whole cell lysate; S: supernatant; I: inclusion bodies.