Impact of the C-terminal clamp-like domain of rpL16 on yeast growth and LSU rRNA processing.

<p><b>A)</b> The wild type (WT) <i>RPL16A</i>, <i>RPL16B</i> yeast strain BY4741 and transformants of yeast strain TY931 expressing the full length allele of <i>RPL16B</i> under control of the GAL1/10 promoter (<i>pGAL-RPL16B</i>), and containing the indicated <i>RPL16B</i>alleles on low copy (lc) plasmids or the FLAG-tagged versions of <i>RPL16B</i> alleles on high copy (hc) plasmids, were grown in selective complete medium with galactose as carbon source (SCGal-U) to an OD₆₀₀ of 0.6 at 30°C. The same amount of cells of each culture was spotted on solid YPD medium in a 1:10 serial dilution series and incubated for 3 days at 30°C. <b>B)</b> and <b>C)</b> strains from <b>A)</b> harbouring the lc plasmids coding for the indicated truncated protein variants of rpL16B were grown at 28°C in SCGal-U and then cultivated in glucose containing medium (YPD). Total RNA was extracted from cells at the indicated time points, and steady-state levels of pre-rRNAs were assayed by northern blotting <b>B)</b>, or primer extension and denaturing gel electrophoresis <b>C)</b>.