Impact of the C-terminal clamp-like domain of rpL16/uL13 on recruitment into early yeast LSU precursors.

<p>Yeast strains Hm653, Hm654 and Hm655 ectopically expressing only the indicated FLAG tagged variants of rpL16/uL13 were grown in selective complete medium with galactose as carbon source and were then cultivated for the indicated times in glucose containing medium. Cells were harvested and subjected to affinity purification using an anti-FLAG matrix as described in Materials and Methods. Wild type yeast strain BY4741 expressing only untagged rpL16/uL13 was included as control in these analyses. The (pre-) rRNA content of the total cellular extracts (“Input” lanes) or of parts of the affinity purified fractions (“IP” lanes) were analyzed by northern blotting using the indicated probes.